The positive charge of DEAE cellulose is due to a protonated amine group. Like all anion exchangers, the resin carries a positive charge that interacts favorably with negative charges. This exchange is utilized to separate proteins that have faintly differing charges. Other derivatives ĭEAE-Sepharose, DEAE-650 and DEAE- Sephadex are commonly used in chromatography.ĭEAE-C is a weak anion exchanger. This procedure is highly suited for transient transfection used for various molecular biology studies. It binds and interacts with negatively charged DNA molecules and via an unknown mechanism brings about the uptake of nucleic acids by the cell. It is added to solution containing DNA meant for transfection. DEAE-D is also used for transfecting animal cells with foreign DNA. DEAE-D ĭEAE- Dextran (DEAE-D) is a positively charged dextran derivative that can be used for vaccine production, gene therapy, protein stabilization, dyslipidemia prevention, flocculating agents, and many other applications. The buffering range for diethanolamine is 8.4-8.8, though the range for DEAE-C varies between manufacturers.

These resins are prepared preswollen although cellulose exchangers swell in a strong basic environment to increase access to binding sites. The proteins are released from the resin by increasing the salt concentration of the solvent or changing the pH of the solution as to change the charge on the protein.ĭEAE-C is synthesized by an alkali-catalyzed reaction of cellulose (obtained from cotton fabric) with 2-chlorotriethylamine, illustrated as following : Types Common resins ĭEAE-C is commonly commercially available as DE52 and DE53. Gel matrix beads are derivatized with diethylaminoethanol (DEAE) and lock negatively charged proteins or nucleic acids into the matrix. Schematic structure of DEAE-C: positively charged diethylaminoethanol groups can bind negative ionsĭiethylaminoethyl cellulose ( DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.

Capto q binds to negative proteins